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The extracts were centrifuged at 10,000 rpm for 10 min at 4 C, and the supernatant was collected and used immediately for the assay at a dilution of 1:10 in PBS/EDTA

Ramirez-[43] studied ergothioneine uptake in six countries (four European countries, Japan, and the USA), and [6] converted these data to average daily values

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